Accession Number : AD0747623

Title :   Cytological Study on the Combined Effect of Olivomycin and Radiation on the Culture of Tumoral HeLa Cells,

Corporate Author : FOREIGN TECHNOLOGY DIV WRIGHT-PATTERSON AFB OHIO

Personal Author(s) : Smertenko,I. I. ; Rudneva,S. V. ; Bolotova,N. P.

Report Date : 23 JUN 1972

Pagination or Media Count : 15

Abstract : It was shown that 0.05 to 1 microgram per ml of olivomycin lowered the amount of living HeLa cell up to 70.5 to 9.7 per cent, as compared to the control. At the same time morphology of the cell layer was impaired and the inhibition coefficient of the mitotic activity was increased up to 16.7-98.9 per cent. In long-time experiments with cloning, clear correlation between the cell survival and the drug dose was noticed. The methods used provided estimation of both results of the combined used of olivomycin and radiation, that is an indirect effect (percentage of the living cells, mitotic activity, number of pathological mitoses, cytological changes) and late evidences (cloning showing the fate of 12 to 14 generations of a single cell). The estimation demonstrated, that the combination had a significantly greater damaging effect on the tumor cells than every component used alone. (Author)

Descriptors :   (*ANTIBIOTICS, *NEOPLASMS), (*TISSUE CULTURE CELLS, NEOPLASMS), (*RADIOTHERAPY, NEOPLASMS), CYTOLOGY, GAMMA RAYS, MITOSIS, PATHOLOGY, CYTOCHEMISTRY, CHEMOTHERAPEUTIC AGENTS, USSR

Subject Categories : Medicine and Medical Research
      Pharmacology

Distribution Statement : APPROVED FOR PUBLIC RELEASE