Accession Number : AD0749461

Title :   Blood Preservation Solutions. XI. Raising the pH to Improve Hemoglobin Function.

Descriptive Note : Progress rept.,

Corporate Author : ARMY MEDICAL RESEARCH LAB FORT KNOX KY

Personal Author(s) : Dawson,R. Ben , Jr. ; Camp,Frank R. , Jr. ; Conte,Nicholas F.

Report Date : 14 APR 1972

Pagination or Media Count : 9

Abstract : The effects of pH increase on hemoglobin function in a standard blood preservative solution during blood storage were assessed. Established methods were used for collecting and storing blood under blood banking conditions, assaying red cell 2,3-diphosphoglycerate (2,3-DPG)--a measure of hemoglobin function, and adenosine triphosphate (ATP)--a measure of red cell viability, as developed previously in this laboratory. Increasing the pH by small gradual increments of the commonly used preservative, acid-citrate-dextrose (ACD), results in a significant improvement in hemoglobin function during the first week of liquid storage at 4 C. Better maintenance of hemoglobin function, as determined by red cell 2,3-DPG concentrations, was observed with preservatives at higher pH levels. On the other hand, at the pH ranges employed in this study, ATP levels were not greatly different over the storage period considered. (Author)

Descriptors :   (*BLOOD, PRESERVATION), (*HEMOGLOBIN, PH FACTOR), METABOLISM, ERYTHROCYTES, VIABILITY, STORAGE, BIOCHEMISTRY, BLOOD TRANSFUSION, ADENOSINE PHOSPHATES

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE