Accession Number : AD0750637

Title :   The Effect of pH and Inosine on the Regeneration of ATP, 2,3-DPG and P(50) of Blood Incubated at 1C. and 37C.

Descriptive Note : Annual summary rept. no. 69, 16 Oct 71-30 Sep 72,

Corporate Author : BRYN MAWR HOSPITAL PA

Personal Author(s) : Strumia,Paul V.

Report Date : 01 OCT 1972

Pagination or Media Count : 9

Abstract : The technical objective of the work reported was to develop practical methods to maintain the highest level of 2,3-DPG and oxygen dissociation along with a reasonable survival. ATP values of 69% plus or minus 2 compatible with 70% survival were achieved with any of the methods reported. Best function, as reflected by 2,3-DPG and P(50) values, was achieved when blood was collected into ACD pH 5.6 and incubated at 37C. with inosine at pH 7.0 or 7.2. (Author)

Descriptors :   (*BLOOD, PRESERVATION), (*ORGANIC PHOSPHORUS COMPOUNDS, BLOOD), STORAGE, TEMPERATURE, PH FACTOR, ADENOSINE PHOSPHATES, BLOOD TRANSFUSION, STABILITY, OXYGEN, ERYTHROCYTES, VIABILITY

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE