Accession Number : AD0750682

Title :   An Automated PCO2 Assay for Glutamic Acid Decarboxylase.

Descriptive Note : Technical note,

Corporate Author : ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD

Personal Author(s) : Zeman,G. H. ; Sobocinski,P. Z. ; Chaput,R. L.

Report Date : JUL 1972

Pagination or Media Count : 15

Abstract : An automated assay for determination of glutamic acid decarboxylase activity is described. The PCO2 of an incubation medium is used as a measure of total CO2 formed by enzymic decarboxylation of glutamate. Details of manifold construction, reagent composition, and data concerning the reliability of the method are presented. (Author)

Descriptors :   (*ENZYMES, *GLUTAMIC ACID), CARBON DIOXIDE, REACTION KINETICS, CHEMICAL ANALYSIS, AUTOMATION, PRESSURE, MEASUREMENT

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE