Accession Number : AD0754823

Title :   Rapid Mixed Leukocyte Test with 3H-Acridine Orange1.

Corporate Author : CALIFORNIA UNIV SAN FRANCISCO MEDICAL CENTER

Report Date : NOV 1972

Pagination or Media Count : 11

Abstract : The report concerns search for a tritium labeled compound which would maximally label lymphocytes in mixed culture (MLC) within 2 - 24 hours. For this purpose tritiated acridine orange (3H-AO) was tested. Interest in acridine orange (AO) stemmed from the report of J. Andersson et al, that by cytophotofluorometric methods, an increase in fluorescence intensity of fixed and AO-stained lymphocytes can be observed 1-3 hours after human allogeneic lymphocytes have been committed to mixed culture. No such increase occurred in lymphocytes from donors compatible at the major histocompatibility locus (HL-A). Data confirms that of Andersson et al., but by a method using liquid scintillation spectrophotometric measurement of 3H-acridine orange binding which is more available than estimation of bound acridine orange by spectrofluorometric fluorescence. Also, a method is described for the preparation of a pure tritiated acridine orange of high specific radioactivity.

Descriptors :   (*LYMPHOCYTES, IN VITRO ANALYSIS), (*LEUKOCYTES, IN VITRO ANALYSIS), LABELED SUBSTANCES, TRITIATED COMPOUNDS, ACRIDINES, DYES, CYTOLOGY, IMMUNOLOGY, FLUORESCENCE, HISTOLOGICAL TECHNIQUES

Subject Categories : Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE