Accession Number : AD0755208

Title :   Isolation of Snake Venom Toxins and Study of Their Mechanism of Action.

Descriptive Note : Final technical rept.,

Corporate Author : ROGOFF-WELLCOME MEDICAL RESEARCH INST PETAH TIQWA (ISRAEL)

Personal Author(s) : DE Vries,Andre

Report Date : MAY 1972

Pagination or Media Count : 48

Abstract : The direct lytic factor (DLF) of cobra venom apparently modifies the red blood cell membrane, activating and eluting membrane-associated enzymes while having no effect on catalase. Several phospholipase A fractions obtained from Vipera palestinae and Naja naja venoms have been characterized (homogeneity, amino acid composition, molecular weight) and shown to possess both phospholipase A and B enzymic activity. Following intravenous injection of Echis coloratus venom, strong positive peroxidase activity can be demonstrated in endothelial cells, basement membrane and sometimes in the surroundings, of mouse brain capillaries and arterioles. A 6.8S immunoglobulin isolated from donkey anti-Russell's viper venom and a 4.68S fraction obtained from it had neutralizing activity against both lethal and coagulating potencies of the venom. The specific neutralizing activities of both Igs were greater than that of the total globulins. (Author)

Descriptors :   (*TOXINS AND ANTITOXINS, VENOMS), (*VENOMS, REPTILES), TOXICITY, ENZYMES, ERYTHROCYTES, MEMBRANES(BIOLOGY), CATALASE, HYDROLASES, HEMOLYSIS, GAMMA GLOBULIN

Subject Categories : Toxicology

Distribution Statement : APPROVED FOR PUBLIC RELEASE