Accession Number : AD0756244

Title :   Cultivation of Hepatitis Virus in Tissue Culture.

Descriptive Note : Annual technical rept. no. 1, 1 Mar 72-28 Feb 73,

Corporate Author : CALIFORNIA UNIV LOS ANGELES DEPT OF MEDICINE

Personal Author(s) : Gitnick,Gary L.

Report Date : 01 MAR 1973

Pagination or Media Count : 12

Abstract : A major step in the cultivation of hepatitis virus and the eventual development of a viral vaccine revolves about the development of appropriate substrates. Hepatitis virus is known to grow rapidly and successfully in liver in vivo. If appropriate tissue culture conditions could be developed, hepatitis virus should grow successfully in liver tissue culture. One problem in attaining this goal has been the rapid development of fibroblastic cells in mixed cultures resulting in the inhibition of growth of hepatocytes. Newer tissue culture techniques have been employed using liver cell homogenates cloned at high dilutions so that hepatocytes alone are planted in tissue culture. Another promising technique employs the cultivation of liver explants grown on plastic plates. This method uses collagenase as a digesting agent and also a medium free of argenine to suppress the growth of nonparenchymal cells. A third tissue culture method involves the use of explant cultures of human liver in which fragments of fetal liver are implanted on polyethylene discs to form monolayer cells. These monolayers are then subcultured and implanted in plastic falcon flasks, Each of these methods has successfully resulted in hepatocyte cultures. (Author Modified Abstract)

Descriptors :   (*INFECTIOUS HEPATITIS VIRUS, TISSUE CULTURE), TISSUE CULTURE CELLS, LIVER, GROWTH(PHYSIOLOGY), CULTURE MEDIA, VACCINES

Subject Categories : Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE