Accession Number : AD0756985

Title :   Flight Muscle Triosephosphate Isomerase of the Mosquito, 'Aedes aegypti', and the Housefly, 'Musca domestica',

Corporate Author : ALBERTA UNIV EDMONTON DEPT OF ENTOMOLOGY

Personal Author(s) : Chiang,Peter K.

Report Date : 13 APR 1972

Pagination or Media Count : 24

Abstract : Triosephosphate isomerase has been purified from thoracic muscle of the mosquito, Aedes aegypti, and also from thoracic muscle of the housefly, Musca domestica. The estimated molecular weight for trisephosphate isomerase from the mosquito and housefly is 60,000. With dihydroxyacetone phosphate as substrate, triosephate isomerase from both sources shows sigmoidal kinetics. For the mosquito triosephosphate isomerase, the apparent Km is I.I mM dihydroxyacetone phosphate. With glyceraldehyde-3-phosphate as substrate, the housefly triosephosphate isomerase activity can be described by the simple Michaelis-Menton equation, except for the occurrence of substrate inhibition. The Km is I.I6 mM for the housefly. The mosquito triosephosphate isomerase can be inhibited by various phosphates, dicarboxylic acids, and arsenate. Insect trisephosphate isomerase is possibly a regulatory enzyme. (Author)

Descriptors :   (*ISOMERASES, *INSECTS), (*SACCHARIDE PHOSPHATES, ISOMERASES), (*ENZYMES, INSECTS), AEDES, MUSCA, DIPTERA, CULICIDAE, MUSCLES, BIOCHEMISTRY, ENTOMOLOGY

Subject Categories : Biochemistry
      Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE