Accession Number : AD0758535

Title :   Structural Studies on the Specific Type VII Pneumococcal Polysaccharide,

Corporate Author : NATIONAL RESEARCH COUNCIL OF CANADA OTTAWA (ONTARIO) DIV OF BIOLOGICAL SCIENCES

Personal Author(s) : Chaudhari,A. S. ; Bishop,C. T. ; Fielder,R. J.

Report Date : 09 JUN 1972

Pagination or Media Count : 12

Abstract : The specific type VII pneumococcal polysaccharide was isolated from the crude capsular material by precipitative and chromatographic methods. It contained D-galactose, D-glucose, L-rhamnose, 2-acetamido-2-deoxy-D-glucose, and 2-acetamido-2-deoxy-D-galactose in the molar ratio of 3.5:2.3:3.0:2.1:1.0. Some of its structural features were revealed by methylation studies, time-lapse hydrolysis, periodate oxidation, and enzymic hydrolysis. The polysaccharide is branched at residues of D-galactose and 2-acetamido-2-deoxy-D-galactose. Non-reducing end groups consisted of D-galactopyranose and 2-acetamido-2-deoxy-D-glucopyranose residues, with the former predominating. Major components of the linear chains were (1 yield 3)-linked L-rhamnose and (1 yield 4)-linked D-glucose; the minor ones were (1 yield 2)-linked L=rhamnose, (1 yield 6)-linked D-galactose, and (1 yield 6)-linked 2-acetamido-2-deoxy-D-glucopyranose. The (1 yield 4)-linked D-glucose components may be present as cellobiose residues. The results are in accord with structural features deduced from the serological cross-reactivity of this polysaccharide. (Author)

Descriptors :   (*DIPLOCOCCUS, POLYSACCHARIDES), (*POLYSACCHARIDES, MOLECULAR STRUCTURE), BACTERIA, IMMUNOLOGY, BIOCHEMISTRY

Subject Categories : Biochemistry
      Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE