Accession Number : AD0760739

Title :   The Influence of Ionic Strength on the Interaction of Quaternary Drugs with Mammalian Acetylcholinesterase in Relation to Possible Regulatory Effects,

Corporate Author : DEFENCE STANDARDS LABS MARIBYRNONG (AUSTRALIA)

Personal Author(s) : Crone,H. D.

Report Date : 09 AUG 1972

Pagination or Media Count : 13

Abstract : The effects of inorganic salts, gallamine trietiodide and (+)-tubocurarine chloride on mammalian acetylcholinesterase (AChE) were examined. The results were obtained mainly from soluble erythrocyte AChE; particle-bound and detergent-solubilized rat brain enzymes were also used. Three aspects of AChE were examined, namely direct effects on activity, the recovery of activity of the diethylphosphorylated enzyme and thirdly the aggregation of the enzyme at low ionic strength as shown by chromatography on columns of Sepharose 6B. The action of gallamine on AChE was controlled by the substrate concentration and the ionic strength of the medium. Both inhibition and activation by gallamine could be observed, depending on the particular conditions used. All effects of gallamine disappeared when the ionic strength was raised to 0.15. It was concluded that the mammalian enzymes closely resembled electric eel AChE. The possible methods by which regulation of AChE activity could occur are discussed in relation to these results. (Modified author abstract)

Descriptors :   (*ACETYLCHOLINESTERASE, *SKELETAL MUSCLE RELAXANTS), CURARE ALKALOIDS, SALTS, HALIDES, ENZYMES, AUSTRALIA

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE