Accession Number : AD0762072

Title :   Interferon and an Interferon Antagonist.

Descriptive Note : Final rept. 1 Nov 68-31 Oct 70,

Corporate Author : MIAMI UNIV CORAL GABLES FLA DEPT OF MICROBIOLOGY

Personal Author(s) : Beasley,Annie R. ; Sigel,M. Michael

Report Date : 31 OCT 1970

Pagination or Media Count : 34

Abstract : Previous research has found crude Newcastle disease virus (NDV) - induced interferon produced in L-929 cell cultures to be highly effective in protecting these cells against Mengovirus but totally incapable of protecting mouse-passaged Ehrlich ascites tumor (EAT) cells. This anamolous intraspecies specifity was due to the presence in the crude material of an interferon antagonist. Adsorption on CM Sephadex C25 and elution with a rising pH gradient effected isolation of semipurified interferon, fully protective of EAT cells, as well as separation of the antagonist. The latter eluted in two positions - one just after the loading volume and the other just prior to interferon elution. This report further characterizes the antagonist by biochemical and biological parameters.

Descriptors :   (*BIOLOGICAL PRODUCTS, INHIBITION), VIRUSES, TISSUE CULTURE CELLS

Subject Categories : Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE