Accession Number : AD0767784

Title :   Studies on Mammalian and Human Pyruvate and alpha-Ketoglutarate Dehydrogenation Complexes.

Descriptive Note : Annual rept. no. 12, 15 Mar 72-14 Mar 73,

Corporate Author : NAGASAKI UNIV (JAPAN) ATOMIC DISEASE INST

Personal Author(s) : Koike,Masahiko

Report Date : 13 AUG 1973

Pagination or Media Count : 26

Abstract : Enzyme systems that catalyze a coenzyme A- and nicotinamide adenine dinucleotide-linked oxidative decarboxylation of pyruvate and 2-oxoglutarate in mammals have been isolated from the Keilin-Hartree preparation of the pig heart muscle as soluble multienzyme complexes with molecular weights in the millions. These multienzyme complexes have distinctive structures and catalyze a coordinated sequence of reaction. The aims of this research are to resolve these complexes into their essential enzymes, to characterize the latter and to determine the composition, macromolecular structure and regulatory mechanism of these two multienzyme complexes. (Modified author abstract)

Descriptors :   (*OXIDOREDUCTASES, MAMMALS), (*PYRUVATES, OXIDOREDUCTASES), (*GLUTARIC ACID, OXIDOREDUCTASES), TRANSFERASES, HUMANS, DEHYDROGENATION, ENZYMES, MOLECULAR STRUCTURE, CHEMICAL ANALYSIS, ELECTROPHORESIS, CHROMATOGRAPHIC ANALYSIS, BIOCHEMISTRY, ESCHERICHIA COLI, JAPAN

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE