Accession Number : AD0767859

Title :   Acetylcholinesterase: Specific Inactivation of Allosteric Effects by a Water-Soluble Carbodiimide,

Corporate Author : BRITISH COLUMBIA UNIV VANCOUVER FACULTY OF PHARMACEUTICAL SCIENCES

Personal Author(s) : Roufogalis,Basil D. ; Wickson,Virginia M.

Report Date : 11 DEC 1972

Pagination or Media Count : 3

Abstract : The aim of the study is to investigate the effects of chemical modification of acetylcholinesterase with specific amino acid reagents and potential affinity alkylating agents. In this way the authors hope to obtain further evidence for the presence of allosteric sites on the enzyme, and to characterize their function and chemical nature. Reported is the use of a water-soluble carbodiimide to specifically inhibit allosteric activation of acetylcholinesternase. This has been achieved without dimishing the basal velocity of hydrolysis of acetylcholine, the enzyme's physiological substrate.

Descriptors :   (*ACETYLCHOLINESTERASE, EXCITATION), INHIBITION, ENZYMES, ALKYLATION, AMINO ACIDS

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE