Accession Number : AD0768695

Title :   A Miniculture Cytotoxicity Assay System Measurement of Cell Number and Viability by Laser Cytograf.

Descriptive Note : Technical rept. Jul 72-Mar 73,

Corporate Author : EDGEWOOD ARSENAL ABERDEEN PROVING GROUND MD

Personal Author(s) : Garry,Vincent F. , Jr. ; Moore,Robert D.

Report Date : SEP 1973

Pagination or Media Count : 15

Abstract : A multiculture system employing confluent monolayers of embryonic human skin fibroblasts has been developed for the purpose of in vitro cytotoxic screening of chemical materials. The parameters of observation used in these experiments are cell number and cell viability as measured in trypan blue dye exclusion. These measurements were automated by the use of the Model 6301 laser Cytograf. (Modified author abstract)

Descriptors :   (*CELLS(BIOLOGY), CHEMICALS), (*CHEMICALS, TOXICITY), DRUGS, VIABILITY, MORPHOLOGY(BIOLOGY), TISSUE CULTURE CELLS, IN VITRO ANALYSIS

Subject Categories : Toxicology

Distribution Statement : APPROVED FOR PUBLIC RELEASE