Accession Number : AD0768737

Title :   Studies of the Mammalian Brain Function in vitro.

Descriptive Note : Annual rept. no. 7, Dec 70-Dec 72,

Corporate Author : KOBE UNIV (JAPAN) DEPT OF PHYSIOLOGY (2ND)

Personal Author(s) : Suda,Isamu

Report Date : DEC 1972

Pagination or Media Count : 24

Abstract : Effects of long term (7 1/4 and 5 3/4 years) storage of the cat in frozen state at -20C in vitro after employing a glycerol perfusion technique were investigated from modes of neuroelectrical activity and histological evidences. The following conclusions were obtained: Glycerol, even if administered via vascular systems, showed cryoprotective effects on the brain tissues as was the case of single cells; it was possible to preserve viability of nervous tissues against many years storage in frozen state; reperfusion for revival brought about enlargement of micro-clefts in tissues which might have been produced by thawing of long term frozen tissues. This may be a cause of bleeding and activity defect in the revived brain; integrated neuroelectrical activity such as brain waves and evoked potentials may be affected significantly by dissolution of functions resulted from disruption in tissues; individual nerve cell activity, on the contrary, may be preserved well essentially or augmented in activity by degenerative processes. (Author)

Descriptors :   (*BRAIN, FREEZING), (*ELECTROPHYSIOLOGY, BRAIN), IN VITRO ANALYSIS, PHYSIOLOGY, CENTRAL NERVOUS SYSTEM, TISSUES(BIOLOGY), CELLS(BIOLOGY), STORAGE, VIABILITY, HISTOLOGY, GLYCEROLS

Subject Categories : Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE