Accession Number : AD0768957

Title :   Pflanzen als Naehrsubstrat fuer Cryptococcus Neoformans (Plants as a Substratum for Growth of Cryptococcus Neoformans),

Corporate Author : ROBERT KOCH-INSTITUT BERLIN (WEST GERMANY)

Personal Author(s) : Staib,F.

Report Date : 22 APR 1971

Pagination or Media Count : 11

Abstract : The suitability of plants as a possible substratum for growth (in vitro) of C. neoformans has been investigated. A sample of hay collected from Wurzburg region and of stems and leaves of dandelion (Taraxacum officinale) were placed in test tubes. One lot of these was sterilized by autoclaving and the second left unsterile. The samples were inoculated by flooding them with suspensions prepared from two known strains of C. neoformans. The suspensions were decanted off and two ml of sterile distilled water were added to each of the inoculated test tubes which were incubated at 26C for one month and subsequently at room temperature. Discrete colonies of C. neoformans appeared after five days on samples of hay as well as of dandelion. They showed on dandelion a characteristic brown colour as described on niger-seed-(Guizotia abyssinica) medium, and their consistency varied with the strain of C. neoformans. Dry colonies of the fungus on infected plants were found to be still viable when tested after keeping for four years at room temperature, and these showed morphological and biochemical characteristics typical of the strains. (Modified author abstract)

Descriptors :   (*YEASTS, PLANTS(BOTANY)), (*CRYPTOCOCCUS, *PLANTS(BOTANY)), FUNGI, GROWTH(PHYSIOLOGY), CULTURE MEDIA, WEST GERMANY

Subject Categories : Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE