Accession Number : AD0769182

Title :   Transfer RNA Methylases from Rat Liver Nuclei,

Corporate Author : ROBERT KOCH-INSTITUT BERLIN (WEST GERMANY)

Personal Author(s) : Kahle,Peter ; Hoppe-Seyler,Peter ; Kroeger,Hans

Report Date : 31 MAR 1971

Pagination or Media Count : 9

Abstract : The intracellular localization of transfer RNA methylases has been investigated. In contrast to previous findings with nuclei from aqueous rat liver homogenates, non-aqueous tissue fractionation results in nuclei with a methylating activity 30 times higher than the corresponding cytoplasm. During fractionation, methylating activity rises parallel to the DNA content of the fraction. Methylases from nonaqueous nuclei and those known from the supernatants of aqueous homogenates have the same substrate specificity. It is concluded that the bulk of tRNA methylases is located within the nucleus of the cell.

Descriptors :   (*Ribonucleic acids, *Liver), (*Enzymes, Ribonucleic acids), Laboratory animals, Cells(Biology), Rats, Nuclei, Experimental data, Cytology, West Germany

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE