Accession Number : AD0769949

Title :   Mechanism of Action of Antigens.

Descriptive Note : Annual progress rept. no. 1,

Corporate Author : PITTSBURGH UNIV PA SCHOOL OF MEDICINE

Personal Author(s) : Gill,Thomas J. , III

Report Date : 15 OCT 1973

Pagination or Media Count : 13

Abstract : The major objective is to define the interaction of poly(Glu52Lys33Tyr15) with the lymphocytes from the highly responding ACI strain of inbred rats and from the poorly responding F344 strain. This will provide information about the interaction of the antigen with the immunocompetent cell by an approach using both a genetic and chemical methods. Techniques have been developed for isolating pure plasma membranes from thymic and splenic lymphocytes. We are syudying the structure of normal membrane and will begin studying membranes from immunized animals soon. Chemicals and enzymatic analyses of the membrane and radioactive labeling of the external components have been performed. There are approximately 25 major components of the lymphocyte membrane whose molecular weights range from 180,000 to 10,000 dalton. There are four major external glycoproteins on both thymic and splenic lymphocytes in the normal rat whose molecular weights re: 2000,000, 150,000, 130,000 and 8,000 daltons. In addition, thymic lymphocytes have a prominent surface glycoprotein 0f 25,000 daltons. Two species of membrane-bound immunoglobulins (130,000 and 2000,000 daltons) and the normal serum immunoglobulin (150,000 daltons) are present on both cell types, although markedly less on thymic than on splenic membranes. (Modified author abstract)

Descriptors :   *Antigens, *Immunology, Cells(Biology), Lymphocytes, Membranes(Biology), Biochemistry, Physiology, Genetics

Subject Categories : Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE