Accession Number : AD0770376

Title :   Yeast Phenylalanyl Transfer Ribonucleic Acid Synthetase: Purification and Complex Formation with Phenylalanine Transfer Ribonucleic Acid.

Descriptive Note : Technical rept. Nov 72-May 73,

Corporate Author : EDGEWOOD ARSENAL ABERDEEN PROVING GROUND MD

Personal Author(s) : Lanks,Karl W. ; Eng,Robert K. ; Baltusis,Liama

Report Date : NOV 1973

Pagination or Media Count : 25

Abstract : It has been found that a homogeneous preparation of phenylalanyl aminoacyl- tRNA synthetase (phe-synthetase) from yeast can be obtained if phenylmethylsulfonyl fluoride is used in all buffers and if phosphocellulose chromatography is employed as a terminal step in the purification. Molecular weight was determined, and complexes between phe-synthetase and tRNA(Phe) were observed in the analytical ultracentrifuge. (Modified author abstract)

Descriptors :   *Enzymes, *Ribonucleic acids, *Nucleoproteins, Molecular weight, Yeasts, Complex compounds, Purification, Chemical reactions, Synthesis(Chemistry), Stability, Proteins(Conjugated)

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE