Accession Number : AD0771924

Title :   A Semiautomated Technique for the Determination of Vitamin C (Ascorbic Acid) in Serum or Plasma Samples,


Personal Author(s) : Goad,W. C. ; Skala,J. H. ; Harding,R. S. ; Sauberlich,H. E.

Report Date : SEP 1973

Pagination or Media Count : 22

Abstract : In nutrition surveys and field studies, large numbers of plasma or serum samples are collected which require measurements for vitamin C (ascorbic acid). Under such conditions, it is seldom possible to perform immediate sample analysis. A method for the determination of serum (plasma) ascorbic acid has been adapted from previously published procedures to meet the needs of such nutrition surveys. The method involves a sample stability preparation on location in the field with the colorimetric quantitation being performed at a later more convenient date employing automated techniques. An evaluation of the procedure indicates that the serum extracts prepared in the field are sufficiently stable (approximately 3%) loss at two weeks), while frozen unstabilized serum (plasma) is completely unsatisfactory. The method is reproducible and sensitive and requires only 0.2 ml or less of a serum (plasma) sample. With proper handling of the samples, the system will rapidly and accurately accommodate large numbers of survey samples requiring vitamin C analysis. (Author)

Descriptors :   *Ascorbic acid, *Blood chemistry, *Vitamins, Colorimetric analysis, Quantitative analysis, Blood serum, Blood plasma, Chemical analysis, Automation, Nutrition

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE