Accession Number : AD0772472

Title :   Solute Radical Inactivation of the Sulfhydryl Enzyme Papain - Sensitization and Protection,

Corporate Author : CALGARY UNIV (ALBERTA) BIOPOLYMER RESEARCH GROUP

Personal Author(s) : Gaucher,G. M. ; Mainman,Barbara L. ; Armstrong,D. A.

Report Date : 12 JAN 1973

Pagination or Media Count : 6

Abstract : Particularly pure N2O-purged papain solutions were irradiated at 0C for different times with 60Co gamma-rays, in the presence of 10-30 mM solutions of acetate, isobutanol, and thiocyanate. Calculated inactivation probabilities per papain-radical reaction are reported in situ protection of both N2-purged and N2O saturated papain solutions by cysteine was found to occur. (Modified author abstract)

Descriptors :   *Papain, *Inactivation, *Free radicals, *Sensitizing, Butanols, Irradiation, Gamma rays, Thiocyanates, Acetates, Canada, Enzymes

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE