Accession Number : AD0774286

Title :   The Effect of Multiple Immunization on Interferon Production.

Descriptive Note : Annual summary rept. no. 1, 1 Nov 72-31 Oct 73,


Personal Author(s) : Beasley,Annie R. ; Sigel,M. Michael

Report Date : 11 JAN 1974

Pagination or Media Count : 20

Abstract : The purpose of this work was the determination of the effects of various immunization regimens on the capacities of lymphocytes to respond to specific and nonspecific stimuli by blastogenesis and interferon production. Healthy young adult volunteers were immunized with single or multiple vaccines, sequential blood samples were obtained and lymphocytes were cultured with and without stimuli. After appropriate incubation periods, uptake of tridiated thymidine was determined as an indication of blastogenesis and supernate fluids were harvested for interferon assays. Emphasis was necessarily placed upon standardization of test procedures, as the potency of the stimuli and the cellular immunity of the volunteers were unknown. Optimal stimulating dosages of phytohemagglutinin and of mumps and influenza antigens have been determined, and interferon assays are in progress. Since the studies to date are preliminary and the study group was small, no conclusions can be drawn but definitive investigations can now be undertaken. (Modified author abstract)

Descriptors :   *Immunization, *Antiviral agents, *Lymphocytes, Blood cells, Immunology, Vaccines, In vitro analysis

Subject Categories : Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE