Accession Number : AD0774579

Title :   Relationship Between Dye Binding and Template Activity of Isolated Avian Chromatin,

Corporate Author : NATIONAL RESEARCH COUNCIL OF CANADA OTTAWA (ONTARIO) DIV OF BIOLOGICAL SCIENCES

Personal Author(s) : Seligy,V. L. ; Lurquin,P. F.

Report Date : 30 OCT 1972

Pagination or Media Count : 3

Abstract : One of the things which has to be ascertained in the context of both the structure and function problems of isolated interphase chromatin is whether the chromatin-associated DNA which is available for in vitro transcription and other enzyme activities is truly free or is covered (masked). Recently, the DNA intercalative dyes actinomycin D and ethidium bromide, whose mechanisms of intercalation are different--actinomycin D binds in the minor groove of DNA with a GpC dependency whereas ethidium bromide binds to the DNA through the major groove with no apparent base requirement--have been shown to be efficacious probes of the DNA in chromatin. The results of this study support the recent findings of Frenster who has shown that acridine orange, which binds in a manner similar to ethidium bromide, is preferentially localized in euchromatin-DNA (long regarded as the active template fraction of the total interphase chromosome).

Descriptors :   *Deoxyribonucleic acids, *Complex compounds, Chemical bonds, Dyes, Molecular structure, Chromosomes, Canada

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE