Accession Number : AD0775034

Title :   The Effects of Maleylation on the Properties of Concanavalin A,

Corporate Author : NATIONAL RESEARCH COUNCIL OF CANADA OTTAWA (ONTARIO) DIV OF BIOLOGICAL SCIENCES

Personal Author(s) : Young,N. Martin

Report Date : 19 JUN 1973

Pagination or Media Count : 7

Abstract : The major product resulting from treatment of concanavalin A with maleic anhydride is a stable, dimeric species, devoid of subunits with broken peptide chains. For complete dissociation of the concanavalin A tetramer, at least eight maleyl groups per subunit were required. The binding site in the fully modified protein was shown to be essentially unchanged, by equilibrium dialysis experiments with para-nitro-phenyl-alpha-D-glucoside. Precipitation reactions with dextran were altered, and would only proceed in the presence of high salt concentration at acid pH. Affinity chromatography on Sephadex G-75 showed that the maleylated dimer behaved in a similar manner to the dimeric form of concanavalin A. (Author)

Descriptors :   *Proteins, Chemical properties, Chemical analysis, Canada

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE