Accession Number : AD0777001

Title :   Effect of DEAE-Dextran and Dimethylsulfoxide on the Results of Titration of Parainfuenza, Respiratory Syncytial and Rhinoviruses in Cell Cultures,

Corporate Author : ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FREDERICK MD

Personal Author(s) : Nisevich,L. L. ; Konstantinova,L. A. ; Stakhanova.,V. M.

Report Date : 1974

Pagination or Media Count : 9

Abstract : In the past few years there have appeared many studies devoted to increasing the titer of viruses and infection ribonucleic acid (RNA) when titrating with various substances. In earlier studies the present authors pointed out that preliminary treatment of a tissue culture with DEAE-dextran in large concentrations increases the infection titer of parainfluenza viruses, while it has no effect on that of the rhinoviruses. This method, however, is quite laborious, and in addition requires lengthy exposure, as well as repeated careful washing of the tissue culture cells. The present study was devoted to the use of DEAE-dextran and dimethylsulfoxide (DMSO) in low concentrations in the supporting medium.

Descriptors :   *Parainfluenza virus, *Rhinoviruses, *Dextran, *Sulfoxides, Tissue culture cells, Growth(Physiology), Ribonucleic acids, Viruses, Culture media, USSR, Translations

Subject Categories : Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE