Accession Number : AD0778720

Title :   Sensitivity to Hemolysis of Canine and Human Red Cells after Timed Exposure to Solutions of Varying pH.

Descriptive Note : Progress rept.,

Corporate Author : ARMY MEDICAL RESEARCH LAB FORT KNOX KY

Personal Author(s) : Shields,Charles E. ; Zegna,Angelo I.

Report Date : 21 NOV 1973

Pagination or Media Count : 11

Abstract : Subjecting human and dog red cells to the stress of nonphysiological pH was found to lead to rapid hemolysis in the dog cells without a significant effect on the human cells. A variety of incubation temperatures and times was used with relatively little difference and with all canine specimens showing hemolysis by 24 hours. Use of solutions with varying pH demonstrated quick onset of hemolysis in alkaline solutions, and delayed lysis in markedly acid solutions. An overall tendency for the pH to rise regardless of the initial starting level, was observed. Upon reaching the alkaline range, lysis of red cells usually occurred. Lysis in the very low acid range could occur earlier and while still in an acid pH range. The narrowness of the range of pH tolerated by canine red cells was in contrast to the broad range tolerated by human erythrocytes. (Modified author abstract)

Descriptors :   *Hemolysis, *pH factor, *Blood preservation, Erythrocytes, Dogs, Humans, Stress(Physiology), Incubation, Anticoagulants, Acids, Alkalinity, Exposure(Physiology), Sensitivity

Subject Categories : Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE