Accession Number : AD0778722

Title :   Alterations in Oxygen Transport Observed in Stored Blood Drawn from Normal or Chronically Anemic Dogs.

Descriptive Note : Progress rept.,

Corporate Author : ARMY MEDICAL RESEARCH LAB FORT KNOX KY

Personal Author(s) : Shields,C. E. ; Meixner,D. E. ; Bratton,J. F. ; Brooks,D. E. ; DeVenuto,F.

Report Date : 27 NOV 1973

Pagination or Media Count : 11

Abstract : Units of blood were drawn from normal and anemic dogs and stored in acid citrate dextrose for six weeks. The units were sampled at intervals and tested for oxygen dissociation P50 point, pH, acid citrate dextrose, 2,3 diphosphoglycerate levels, and methylene blue uptake, as well as osmotic fragility. The general loss of these with storage paralleled the findings obtained with normal bank blood. One particular exception was the finding of higher 2,3 diphosphoglycerate levels in anemic blood that tended to be higher than normal throughout storage. Anemic cells were also found to be more resistant to osmotic lysis, possibly because of their microcytic shape. The biological response to the degree of chronic anemia induced in these dogs appeared to be primarily an increase in 2,3 diphosphoglycerate level without marked alteration in the oxygen curve or other investigated metabolic factors. (Modified author abstract)

Descriptors :   *Blood preservation, *Anemias, *Blood transfusion, *Blood donors, Hemolysis, Adenosine phosphates, Oxygen, Transportable, Blood banks, Dogs, Physiological effects, Methylene blue, Osmosis, Response(Biology), Metabolism, Erythrocytes

Subject Categories : Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE