Accession Number : AD0780009

Title :   Some Effects of DBcAMP on Rabbit Bone Marrow Cells in Culture: Cell Clumping and Cholinesterase Production.

Descriptive Note : Technical rept. Jul 72-Jul 73,

Corporate Author : EDGEWOOD ARSENAL ABERDEEN PROVING GROUND MD

Personal Author(s) : Garry,Vincent F. , Jr. ; Harris,Larrel W. ; Petrali,John P.

Report Date : APR 1974

Pagination or Media Count : 14

Abstract : Bone marrow cells derived from phenylhydrazine-treated rabbits were grown in vitro for 24 hours. Marrow cultivated in the presence of .001 M dibutyryl 3',5'-cyclic monophosphate (DBcAMP) formed large, dense cell clusters whereas those from control cultures formed smaller, more discrete cell clusters. Actinomycin D inhibited cluster formation. Erythroid cell maturation was also inhibited in the presence of DBcAMP. However, cholinesterase synthesis was enhanced. Most of the cells containing newly synthesized membrane-bound cholinesterase are erythroid. On the basis of the data obtained, it is suggested that the cyclic nucleotide may stimulate membrane protein synthesis. (Author)

Descriptors :   *Bone marrow, *Adenosine phosphates, *Cholinesterase, Biosynthesis, Nucleotides, Enzymes, Cells(Biology), Proteins, Membranes(Biology), Erythrocytes

Subject Categories : Biochemistry
      Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE