Accession Number : AD0781709
Title : The Microbial Degradation of Aromatic Petroleum Products.
Descriptive Note : Annual rept. no. 1, 25 Jun 73-24 Jun 74,
Corporate Author : TEXAS UNIV AUSTIN DEPT OF MICROBIOLOGY
Personal Author(s) : Gibson,David T.
Report Date : 24 JUN 1974
Pagination or Media Count : 70
Abstract : It is the purpose of this contract to investigate the mechanisms used by soil bacteria to oxidize, ortho-, meta- and para-xylene. The progress report describes significant accomplishments including: The isolation of a strain (Pxy) of Pseudomonas that will oxidize p- and m-xylene to carbon dioxide and water; The isolation of a mutant strain of the above organism that accumulates p-toluic acid from p-xylene; The isolation of a second mutant strain that accumulates 3- and 4-methylcatechol from m- and p-xylene; The elucidation of the metabolic sequences used by Pseudomonas pxy to degrade p- and m-xylene to aliphatic products; The isolation and identification of intermediates in the metabolism; The isolation of the first bacterium reported to grow with o-xylene as the sole source of carbon and energy; and, Identification of 3,4-dimethylcatechol as an intermediate in o-xylene metabolism.
Descriptors : *Xylenes, *Pseudomonas, *Metabolism, *Oxidation, Nuclear magnetic resonance, Reaction kinetics, Biodeterioration, Soils, Bacteria
Subject Categories : Biochemistry
Distribution Statement : APPROVED FOR PUBLIC RELEASE