Accession Number : AD0782605

Title :   Bean Mesophyll Protoplasts: Production, Culture and Callus Formation,

Corporate Author : RAYDIST NAVIGATION CORP HAMPTON VA

Personal Author(s) : Pelcher,L. E. ; Gamborg,O. L. ; Kao,K. N.

Report Date : 27 DEC 1973

Pagination or Media Count : 6

Abstract : High yields of viable protoplasts were produced from leaf tissue of Phaseolus vulgaris L. var. Pinto. Consistent production of protoplasts depended on enzymatic liberation of the protoplasts near pH 7.0. Cultured protoplasts regenerated cell walls within 24-48 h after isolation. Cell division was observed within 72 h after isolation and sustained division led to callus formation. (Author)

Descriptors :   *Beans, Cells(Biology), Growth(Physiology), Plants(Botany), Cell division, Cultures(Biology), Canada

Subject Categories : Agronomy, Horticulture and Aquiculture

Distribution Statement : APPROVED FOR PUBLIC RELEASE