Accession Number : AD0782733

Title :   Cultivation of Hepatitis Virus in Tissue Culture.

Descriptive Note : Annual rept. 1 Jul 73-30 Jun 74,

Corporate Author : CALIFORNIA UNIV LOS ANGELES DEPT OF MEDICINE

Personal Author(s) : Gitnick,G. L.

Report Date : 01 JUL 1974

Pagination or Media Count : 9

Abstract : A carefully planned program with the eventual goal, being the development of a live viral vaccine for hepatitis, was undertaken at the UCLA Center for the Health Sciences. The initial phase of this program aimed at the development of tissue culture substrates for use in vaccines. Reproducible and reliable techniques have been developed for the short and long term maintenance of hepatocytes in monolayer tissue culture. Using colagenase in association with an argenine deficient selective medium, fetal and adult hepatocytes have been maintained in fibroblast free cultures for periods of up to 5 months. On subculture, primary cultures maintain their epithelial characteristics. Hepatitis B Antigen has been maintained intracellularly in these cultures for periods of up to 12 days before titers have fallen off. Evidence of replication exists in that these periods, in cell free media, titers fall off at a much more rapid rate. (Modified author abstract)

Descriptors :   *Infectious hepatitis virus, Tissue culture cells, Liver, Growth(Physiology), Culture media, Vaccines

Subject Categories : Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE