Accession Number : AD0784280

Title :   Mechanisms of Hemolysis.

Descriptive Note : Annual summary rept.,

Corporate Author : CALIFORNIA UNIV SAN DIEGO LA JOLLA SCHOOL OF MEDICINE

Personal Author(s) : Bernstein,Eugene F.

Report Date : MAY 1974

Pagination or Media Count : 43

Abstract : Erythrocyte damage by surface damage, shear stress or osmotic lysis was accompanied by the release of free glyceric acid/bis(dihydrogen-phosphate) (DPG) to the plasma in greater concentrations than normally exist within the cell, compared to hemoglobin. Following incubation, DPG was rapidly depleted in both control and damaged specimens, and the oxyhemoglobin dissociation curve was shifted to the left of an appropriate degree. Red blood cell adenosine triphosphate (ATP) concentration was substantially depleted only after osmotic lysis, although free plasma ATP was demonstrable following both shear and osmotically induced injury. Platelet adhesiveness is markedly decreased by surface injury. Platelet aggregation in response to adenosine phosphates, collagen and thrombin is markedly reduced following surface injury. Platelet Factor 3 availability is decreased following surface injury, but not following shear stress, although Platelet Factor 3 content is not significantly altered after either form of damage. (Modified author abstract)

Descriptors :   *Hemolysis, *Erythrocytes, *Blood platelets, Mechanical organs, Medical research, Traumatic shock, Adenosine phosphates, Osmosis, Membranes(Biology), Blood plasma, Experimental data

Subject Categories : Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE