Accession Number : AD0786955

Title :   Affinity Chromatography of Triton-solubilized Brain Acetylcholinesterase,


Personal Author(s) : Dawson,R. M. ; Crone,H. D.

Report Date : 21 JAN 1974

Pagination or Media Count : 6

Abstract : Ox and rat brain acetylcholinesterase, solubilized with Triton X-100, have been purified by affinity chromatography. Gels substituted with two isomeric inhibitors were used. Protein impurities were eluted with NaCl solutions of low ionic strength, and acetylcholinesterase was recovered by increasing the ionic strength or by eluting with the enzyme inhibitor edrophonium chloride. Acetylcholinesterase of low purity was obtained when Triton was not present in the crude preparation and when it was omitted from eluting solvents. A high degree of purification was observed when the surfactant was present throughout the chromatography. (Author)

Descriptors :   *Acetylcholinesterase, *Purification, Brain, Chromatography, Australia

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE