Accession Number : AD0786966

Title :   Ion-exclusion Effects on the Chromatography of Acetylcholinesterase and Other Proteins on Agarose Columns at Low Ionic Strength,

Corporate Author : DEFENCE STANDARDS LABS MARIBYRNONG (AUSTRALIA)

Personal Author(s) : Crone,Hugh D.

Report Date : 10 DEC 1973

Pagination or Media Count : 10

Abstract : Acetylcholinesterase (AChE), serum albumin and other proteins were chromatographed on columns of Sepharose 4B at varied ionic strengths of the eluting buffers, pH 8.4. AChE and serum albumin were progressively excluded from the gel as the ionic strength was lowered, whereas neutral or basic proteins showed no response or were adsorbed to the column. For the two acidic proteins (AChE and albumin) a hyperbolic relationship between K(av) and ionic strength was found, and two empirical constants were calculated to define the relationship. It was concluded that the exclusion effects were due to the repulsion between the anionic proteins and anionic groups on the gel at low salt concentration. (Modified author abstract)

Descriptors :   *Acetylcholinesterase, *Column chromatography, *Serum albumin, Acids, Gels, Proteins, Anions, Australia

Subject Categories : Biochemistry
      Physical Chemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE