Accession Number : AD0806438

Title :   STEROID-PROTEIN INTERACTIONS. V. COMPARISON OF SPECTROPHOTOMETRIC AND EQUILIBRIUM-DIALYSIS PROCEDURES FOR DETERMINATION OF BINDING CONSTANTS,

Corporate Author : ARMY MEDICAL RESEARCH LAB FORT KNOX KY

Personal Author(s) : Westphal, Ulrich ; Ashley, Billy D. ; Selden, George L.

Report Date : 04 AUG 1958

Pagination or Media Count : 13

Abstract : An attempt was made to establish the validity of the spectrophotometric procedure for the determination of interaction between proteins and delta4-3-ketosteroids, this method was to be compared with the equilibrium-dialysis technique. The binding values obtained by the spectrophotometric method agree with those determined independently by equilibrium dialysis. The reduction of the extinction coefficient of an alpha, beta-unsaturated ketone by interaction with proteins provides an adequate basis for the determination of binding values. (Author)

Descriptors :   (*STEROIDS, BIOCHEMISTRY), (*PROTEINS, BIOCHEMISTRY), SPECTROPHOTOMETERS, EQUILIBRIUM(PHYSIOLOGY), ABSORPTION, SERUM ALBUMIN, HORMONES, TESTOSTERONE, PROGESTERONE, CORTICOSTEROID AGENTS, ULTRAVIOLET SPECTROSCOPY, DIALYSIS.

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE