Accession Number : AD0806444

Title :   A COMPARISON OF VARIOUS LIPID EXTRACTION METHODS AND THEIR APPLICATION TO THE DETERMINATION OF TOTAL LIPIDS IN ESCHERICHIA COLI, STRAINS B AND B/R, WHEN GROWN UNDER VARIED ENVIRONMENTAL CONDITIONS,

Corporate Author : ARMY MEDICAL RESEARCH LAB FORT KNOX KY

Personal Author(s) : Woodside, E. E. ; Kocholaty, W.

Report Date : 14 OCT 1958

Pagination or Media Count : 12

Abstract : The purpose of this inquiry was to apply a highly specific microextraction procedure to the determination of the total lipid content of Escherichia coli, strains B and B/r. Several lipid extraction procedures were compared and evaluated. Employing the microextraction method of Hsiao (11) for the determination of total lipids in Excherichia coli, it was found that the lipid contents of E. coli B and B/r, when grown on a mineral medium with acetate as the only carbon source, did not differ significantly. The total lipid content of E. coli showed significant differences depending upon the environmental conditions to which the cells were exposed. A decrease in the total lipid content was observed during the spontaneous dark recovery phase of ultraviolet irradiated cells of E. coli B. (Author)

Descriptors :   (*ESCHERICHIA COLI, RADIATION EFFECTS), (*BACTERIAL EXTRACTS, MICROANALYSIS), ULTRAVIOLET RADIATION, BIOCHEMISTRY, LIPIDS, TEST METHODS, RADIOBIOLOGY.

Subject Categories : Biochemistry
      Radiobiology
      Microbiology
      Pharmacology

Distribution Statement : APPROVED FOR PUBLIC RELEASE