Accession Number : AD0806445

Title :   DETERMINATION OF THE CARBOHYDRATE CONTENT OF ESCHERICHIA COLI, B AND B/R GROWN UNDER VARIOUS ENVIRONMENTAL CONDITIONS,

Corporate Author : ARMY MEDICAL RESEARCH LAB FORT KNOX KY

Personal Author(s) : Woodside, E. E. ; Kocholaty, W.

Report Date : 16 OCT 1958

Pagination or Media Count : 13

Abstract : An investigation was made to study the synthesis and distribution of carbohydrates in cultures of Escherichia coli under various environmental condition. Cells of Escherichia coli, B and B/r, produce pentoses and hexoses when grown on a mineral medium containing acetate as the only source of carbon. The intracellular and extracellular distribution of these carbohydrates vary with the organism considered and with the growth phase of the culture. Cells of Escherichia coli B/r in their logarithmic growth phase produce approximately twice the amount of intracellular hexose as the parent strain, Escherichia coli B. Following the McQuillen and Roberts (15) fractionation procedure, almost 90% of the total intracellular carbohydrate content was accounted for in the cold and hot trichloracetic acid soluble fractions. Following x-irradiation of E. coli B/r, considerable amounts of intracellular pentoses and hexoses are released into the growth medium. (Author)

Descriptors :   (*ESCHERICHIA COLI, RADIATION EFFECTS), CARBOHYDRATES, METABOLISM, CULTURE MEDIA, ACETATES, GROWTH(PHYSIOLOGY), HEXOSES, PENTOSES, BIOCHEMISTRY, X RAYS.

Subject Categories : Biochemistry
      Radiobiology
      Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE