Accession Number : AD0810287

Title :   A PRECISE METHOD FOR REPLICATING SUSPENSION CULTURES OF MAMMALIAN CELLS,

Corporate Author : FORT DETRICK FREDERICK MD

Personal Author(s) : Weirether, Francis J.

Report Date : FEB 1967

Pagination or Media Count : 12

Abstract : A simple, readily assembled shaker culture system for the cultivation of mammalian cells is described and illustrated. It uses no specialized glassware and equipment and consists of an Erlenmeyer flask fitted with a breather-sampling assembly. This unit was employed in quantitating the effects of several variables including medium ingredients, serial transfers, and freezing and storage on two variants of the L cell line. The method is sensitive and reproducible and allows for growth of cells in suspension for extended periods of time. Large numbers of cells can be mass-produced. Many replicates can be run simultaneously to yield data for statistical analysis. (Author)

Descriptors :   (*TISSUE CULTURE CELLS, TEST METHODS), CULTURE MEDIA, GROWTH(PHYSIOLOGY), LABORATORY EQUIPMENT, STATISTICAL ANALYSIS, MAMMALS, REPRODUCTION.

Subject Categories : Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE