Accession Number : AD0819691

Title :   THE STORAGE OF PRIMATE BONE MARROW FOR HOMOLOGOUS TRANSPLANTATION.

Descriptive Note : Final technical rept. 1 Jul 66-30 Jun 67,

Corporate Author : RADIOBIOLOGISCH INSTITUUT TNO RIJSWIJK (NETHERLANDS)

Personal Author(s) : VAN Putten, L. M. ; van Bekkum, D. W. ; Dicke, K. A.

Report Date : JUL 1967

Pagination or Media Count : 13

Abstract : A study was made of the efficiency of storage of Rhesus monkey bone marrow cells for homologous transplantation, as a technical preparation for the future availability of a bank of stored bone marrow cells. A standardized freezing method, utilizing 10% polyvinylpyrrolidone and 10% glycerol as additives to the cell suspensions, had been shown earlier to give about 50% recovery of the protective capacity in irradiated hosts in autologous studies. The same method applied to homologous transplantation failed to give consistent results; in only 5 out of 12 attempts could a take of frozen cells be recorded. The failure to induce hemopoietic repopulation of homologous irradiated recipients were not associated with lower bone marrow cell numbers infused, or with differences in the cell concentrations during storage, or in the radiation doses used, when compared with the successful cases. Although more failures were observed when larger ampoules were used to store the frozen suspensions and when the suspensions were stored for longer periods of time, these conditions cannot account for all of the failures. It is concluded that the failure to obtain the expected results may either be due to technical differences in the method of procuring bone marrow suspensions for homologous compared to autologous transplantation or to a fundamental difference involving the immunogenetic complications of homologous transplantation. The former of these alternatives demands further study in primates; the latter is more suitably elucidated in rodent studies.

Descriptors :   (*BONE MARROW, STORAGE), (*TRANSPLANTATION, BONE MARROW), MONKEYS, PREPARATION, RADIATION INJURIES, FREEZING, WHOLE BODY IRRADIATION, ADDITIVES, GLYCEROLS.

Subject Categories : Anatomy and Physiology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE