Accession Number : AD0822331

Title :   FAILURE TO FIND AN ACTIVATED ARGININE RESIDUE IN THE ACTIVE SITE OF CHYMOTRYPSIN.

Descriptive Note : Technical rept. Mar-Jun 66,

Corporate Author : EDGEWOOD ARSENAL ABERDEEN PROVING GROUND MD

Personal Author(s) : Schaffer, Norwood K. ; Balakir, Richard A.

Report Date : OCT 1967

Pagination or Media Count : 23

Abstract : This report describes an attempt to confirm the presence of an activated arginine residue in an active-site peptide isolated from an enzymic hydrolyzate of isopropyl methylphosphonofluoridate (GB)-inactivated chymotrypsin (IMeP-chymotrypsin) reported by a previous investigator. The present results failed to confirm his finding. Arginine was not found in the peptide either before or after treatment with ammonia. The composition of the peptide was found to be Gly.Asp.IMePSer.Gly.Gly.Pro.Leu. An unknown, basic, ninhydrin-reacting substance was detected after ammonia treatment of the peptide. (Author)

Descriptors :   *AMINO ACIDS), (*CHYMOTRYPSIN, (*CHROMATOGRAPHIC ANALYSIS, CHYMOTRYPSIN), AMMONIA, GLYCINE, ASPARTIC ACID, METHIONINE, PHENYLALANINE, LABELED SUBSTANCES, RADIOACTIVE ISOTOPES, PHOSPHORUS.

Subject Categories : Biochemistry
      Physical Chemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE