Accession Number : AD0825251

Title :   A-BAND OSMIOPHILIA. ITS MICROSCOPIC DEMONSTRATION WITH SILVER.

Descriptive Note : Technical rept.,

Corporate Author : EDGEWOOD ARSENAL ABERDEEN PROVING GROUND MD

Personal Author(s) : Schofield, Brian ; Terner, Jacob Y. ; Lundy, Jacob ; Thompson, John H.

Report Date : NOV 1967

Pagination or Media Count : 18

Abstract : The relatively specific affinity of osmium tetroxide (OsO4) for the A-bands, or myosin-containing areas, of striated and cardiac muscle from goats and rabbits is demonstrated. A silver-methenamine (Ag-Me) reagent was used so that this A-band osmiophilia could be identified by electron and light microscopy. Histochemical reactions indicate that the OsO4 forms a partially reduced complex with the amino and/or guanido groups of the A-band protein through coordinate covalent bonds. This partially reduced complex then reduces the Ag-Me reagent as it itself is oxidized. The reduced silver precipitates along the muscle A-band, giving a selective stain for that muscle component at the electron or light microscopic level. The presence of OsO4 in the A-bands was also verified by reducing the osmium to a black compound with sulfurous acid and phenylenediamine and by forming a red complex of osmium with alpha-naphthylamine. (Author)

Descriptors :   *HISTOLOGICAL TECHNIQUES), (*MUSCLES, (*BIOLOGICAL STAINS, MUSCLES), OSMIUM COMPOUNDS, TETROXIDES, VISUAL INSPECTION, ELECTRON MICROSCOPY, SILVER, MUSCLE PROTEINS, AMINES, MICROSCOPY.

Subject Categories : Biochemistry
      Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE