Accession Number : AD0828886

Title :   THE EFFECT OF PEPTIDE ANTIBIOTICS ON PHAGOCYTOSIS.

Descriptive Note : Final rept. 31 Mar 66-31 Aug 67,

Corporate Author : NOTRE DAME UNIV IN DEPT OF MICROBIOLOGY

Personal Author(s) : Downey, Ronald J.

Report Date : MAR 1968

Pagination or Media Count : 18

Abstract : A phagocytosis-promoting factor (PPF) has been isolated from conventional and germfree guinea pig and rat peritoneal exudate fluid. The factor was found to resemble a globulin type of serum protein. It contained no hemolytic complement or gamma globulin. Electrophoresis under conditions of low pH and histochemical techniques indicated the PPF was a glycoprotein. Divalent cations were required for enhancement produced by the PPF. The terminal sialic acid group of the glycoprotein was shown to be indispensible for opsonic activity. The PPF appears to promote adsorption of bacteria to phagocyte but not ingestion. The PPF was inactive when used with polystyrene latex particles or with heterologous cells. (Author)

Descriptors :   *RETICULOENDOTHELIAL SYSTEM), (*ANTIBIOTICS, GERM FREE ANIMALS, RATS, GUINEA PIGS, GAMMA GLOBULIN, ELECTROPHORESIS, GLYCOPROTEINS, BLOOD SERUM, HISTOLOGICAL TECHNIQUES, STAPHYLOCOCCUS AUREUS, ENZYMES.

Subject Categories : Pharmacology

Distribution Statement : APPROVED FOR PUBLIC RELEASE