Accession Number : AD0835876

Title :   IMMUNOLOGICAL REACTIONS IN VIRAL HEPATITIS.

Descriptive Note : Final technical rept. Dec 66-Nov 67,

Corporate Author : GENOA UNIV (ITALY)

Personal Author(s) : Pernis, Benvenuto ; Tolentino, Paolo

Report Date : DEC 1967

Pagination or Media Count : 21

Abstract : Two different investigations were performed in the field of the immune reactions in viral hepatitis. The first investigation was performed to ascertain, with immuno-fluorescence, the presence of viral or virus-associated antigens in the liver or in the duodenal mucosa of patients in the acute stage of infectious hepatitis. As a source of antibodies the serum from patients convalescent from the disease was employed, and as a method of demonstration of the antigen-antibody reaction the indirect or 'sandwich' technique was used with fluorescein-conjugated antisera directed against the different human immunoglobulins. In the second investigation it was found that the serum from patients in the acute stage of infectious hepatitis effectively inhibits the mitotic activity of human lymphocytes cultivated 'in vitro' with phytohemagglutinin. The inhibiting factor is absent from the serum of patients convalescent from infectious hepatitis or affected by non infectious hepatic diseases. Apparently the acute infectious hepatitis serum does not block the transformation of PHA-stimulated lymphocytes into blast-like cells nor does reduce the percentage of cells that can be shown, by autoradiography, to incorporate 3H-thymidine. Electron microscopy showed elements with the typical structures of 'blasts' in the cultures performed in the presence of acute hepatitis serum; these cells did not contain structures that could suggest the presence of viral particles. (Author)

Descriptors :   (*INFECTIOUS HEPATITIS VIRUS, SERODIAGNOSIS), (*FLUORESCENT ANTIBODY TECHNIQUES, INFECTIOUS HEPATITIS VIRUS), TISSUES(BIOLOGY), LIVER, BIOPSY, HISTOLOGICAL TECHNIQUES, GAMMA GLOBULIN, IMMUNE SERUMS, INTESTINES, ANTIGEN ANTIBODY REACTIONS, IMMUNITY, MITOSIS, INHIBITION, LYMPHOCYTES, ELECTRON MICROSCOPY, ITALY.

Subject Categories : Medicine and Medical Research
      Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE