Accession Number : AD0837453

Title :   RESEARCH ON THE DENATURATION OF DESOXYRIBONUCLEIC ACIDS (DNA),

Corporate Author : ARMY BIOLOGICAL LABS FREDERICK MD

Personal Author(s) : Thomas, R.

Report Date : 01 JUL 1965

Pagination or Media Count : 15

Abstract : The ultraviolet absorption of desoxyribonucleic acids (DNA) in neutral saline solution is much lower than that expected from the sum of the absorptions of its constituents. The observed spectrum can be made to approach the calculated one by applying mild treatments which are presumably without action on phosphoester linkages (pH, temperature and salt concentration changes). The incidence of these facts on the questions of the chemical structure and biological properties of DNA is discussed. The results are interpreted as reflecting a denaturation of the DNA, i.e. the destruction of a secondary molecular structure constituted by labile bonds involving the puric and pyrimidic rings. (Author)

Descriptors :   (*DEOXYRIBONUCLEIC ACIDS, MOLECULAR STRUCTURE), ULTRAVIOLET SPECTRA, VISIBLE SPECTRA, PH FACTOR, TEMPERATURE, SALTS, STEREOCHEMISTRY, NETHERLANDS.

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE