Accession Number : AD0837468
Title : CONFIGURATION AND LONGITUDINAL DISTRIBUTION OF DNA MOLECULES IN SOLUTION,
Corporate Author : ARMY BIOLOGICAL LABS FREDERICK MD
Personal Author(s) : Lang, Dimitrij ; Kleinschmidt, Albrecht K. ; Zahn, Rudolf K.
Report Date : 18 OCT 1965
Pagination or Media Count : 18
Abstract : A diffusion method is described for visualization of DNA molecules which is equivalent to the spreading of a mixed DNA-protein solution. Macromolecules diffusing in a solution of about 5 x 10 to the minus 8th power g DNA per ml are adsorbed by a cytochrome c-film at the air-water interface. Subsequently they are transferred to electron microscopic supports. In the enlarged two-dimensional image the end-to-end distances and the length distribution are measured for different DNA preparations (from cod fish, trout, holothuria and T2 phage). The molecular shape is not a random coil or a rod but intermediate on account of electrostatic interaction. (Author)
Descriptors : (*DEOXYRIBONUCLEIC ACIDS, MOLECULAR STRUCTURE), SOLUTIONS(MIXTURES), ADSORPTION, ELECTRON MICROSCOPY, MORPHOLOGY(BIOLOGY), ELECTROSTATICS, INTERACTIONS, EQUATIONS, DIFFUSION, MOLECULAR WEIGHT, NETHERLANDS.
Subject Categories : Biochemistry
Distribution Statement : APPROVED FOR PUBLIC RELEASE