Accession Number : AD0837468

Title :   CONFIGURATION AND LONGITUDINAL DISTRIBUTION OF DNA MOLECULES IN SOLUTION,

Corporate Author : ARMY BIOLOGICAL LABS FREDERICK MD

Personal Author(s) : Lang, Dimitrij ; Kleinschmidt, Albrecht K. ; Zahn, Rudolf K.

Report Date : 18 OCT 1965

Pagination or Media Count : 18

Abstract : A diffusion method is described for visualization of DNA molecules which is equivalent to the spreading of a mixed DNA-protein solution. Macromolecules diffusing in a solution of about 5 x 10 to the minus 8th power g DNA per ml are adsorbed by a cytochrome c-film at the air-water interface. Subsequently they are transferred to electron microscopic supports. In the enlarged two-dimensional image the end-to-end distances and the length distribution are measured for different DNA preparations (from cod fish, trout, holothuria and T2 phage). The molecular shape is not a random coil or a rod but intermediate on account of electrostatic interaction. (Author)

Descriptors :   (*DEOXYRIBONUCLEIC ACIDS, MOLECULAR STRUCTURE), SOLUTIONS(MIXTURES), ADSORPTION, ELECTRON MICROSCOPY, MORPHOLOGY(BIOLOGY), ELECTROSTATICS, INTERACTIONS, EQUATIONS, DIFFUSION, MOLECULAR WEIGHT, NETHERLANDS.

Subject Categories : Biochemistry
      Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE