Accession Number : AD0838750
Title : TRANSFORMATION IN BACILLUS CEREUS: A CRITIQUE,
Corporate Author : FORT DETRICK FREDERICK MD
Personal Author(s) : Goldberg, Ivan D. ; Gwinn, Darrel D.
Report Date : JUN 1968
Pagination or Media Count : 14
Abstract : A procedure for making Bacillus cereus 569 competent and for the isolation of the 'competence factor' was described by Felkner and Wyss. An examination of the strains used by Felkner and Wyss in their study revealed that they were not B. cereus by the following criteria: They were auxotrophic for tryptophan and could be transduced to prototrophy by phage SP-10 that had been propagated on Bacillus subtilis W-23-S(r); they became competent when grown according to the procedure devised by Anagnostopoulos and Spizizen for B. subtilis and could be transformed to prototrophy with deoxyribonucleic acid (DNA) isolated from B. subtilis W-23-S(r); and when DNA from one of the strains was melted, the melting curve was almost identical to that obtained with the DNA of B. subtilis 168 ind(-). The present studies clearly indicate that the strains used by Felkner and Wyss were not B. cereus but were, in fact, B. subtilis. (Author)
Descriptors : (*BACILLUS CEREUS, TRANSFORMATIONS), GENETICS, BACTERIOPHAGES, DEOXYRIBONUCLEIC ACIDS, TRYPTOPHAN, BACILLUS SUBTILIS.
Subject Categories : Microbiology
Distribution Statement : APPROVED FOR PUBLIC RELEASE