Accession Number : AD0858934

Title :   The Requirement for Coenzyme A in the Phosphoroclastic Reaction of Anaerobic Bacteria.

Descriptive Note : Final rept.,

Corporate Author : MASSACHUSETTS UNIV AMHERST DEPT OF MICROBIOLOGY

Personal Author(s) : Hespell, R. B. ; Joseph, R. ; Moftlock, R. P.

Report Date : 1969

Pagination or Media Count : 27

Abstract : A variety of bacteria which degrade pyruvate by the 'phosphoroclastic reaction' were examined with respect to the role of CoA in this reaction. This strictly anaerobic bacteria, which cleave pyruvate by the phosphoroclastic reaction characteristic of Clostridia, required catalytic levals of CoA for the Co2-pyruvate exchange and acetoin forming portions of the phosphoroclastic reaction. These reactions were reversibly inhibited by the CoA analogue, desulpho-CoA. In contrast, using cell-free extracts of bacteria which degrade pyruvate by the coliform phosphoroclastic reaction (pyruvate formate-lyase), no requirement for CoA could be observed for the formate-pyruvate exchange reaction. It is suggested that CoA serves a regulatory function in the early portion of the clostridal type of phosphoroclastic reaction. (Author)

Descriptors :   *COENZYMES, *CLOSTRIDIUM, INHIBITION, CARBON DIOXIDE, PYRUVATES, CATALYSTS, METABOLISM, CELLS(BIOLOGY).

Subject Categories : Biochemistry
      Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE