Accession Number : AD0860419

Title :   Oxidation-Reduction Potential and Concomitant Growth Patterns of Cultures of Earle's L Cells in Centrifuge Spinner Bottles,

Corporate Author : FORT DETRICK FREDERICK MD

Personal Author(s) : Daniels, William F. ; Garcia, Luis H. ; Rosensteel, John F.

Report Date : SEP 1969

Pagination or Media Count : 18

Abstract : Previous studies of oxidation-reduction potential (ORP) variation in monolayer (Roux bottle) cultures pointed out the need for data on pH and ORP patterns in simple spinner cultures. This information was desirable for optimizing conditions of growth in small 1-liter and 7.5-liter New Brunswick fermentors. Results of experiments in 250-ml centrifuge spinner vessels show that incubation of media prior to inoculation induces desirable qualities reflected in better growth. The importance of initial ORP values of the medium is discussed. The relationship of ORP levels to yield and longevity of cell growth is also considered. The ORP level of the medium at inoculation is shown to be affected by previous incubation. (Author)

Descriptors :   (*TISSUE CULTURE CELLS, OXIDATION REDUCTION REACTIONS), GROWTH(PHYSIOLOGY), TISSUE CULTURE, CELLS(BIOLOGY), VIABILITY, PH FACTOR, ELECTRODES.

Subject Categories : Anatomy and Physiology
      Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE