Accession Number : AD0860651

Title :   Studies on Microbial Degradation of Toxic Phosphorus Esters. Part 1. Detoxification of O,O-Dimethyl-O-(2-carbomethoxy-1-methylvinyl) Phosphate by Soil Bacteria.

Descriptive Note : Interim rept.,

Corporate Author : AIR FORCE ARMAMENT LAB EGLIN AFB FL

Personal Author(s) : Wolverton, B. C. ; Madura, Carolyn

Report Date : SEP 1969

Pagination or Media Count : 19

Abstract : In an attempt to find a new method for detoxifying cholinesterase-inhibiting, organophosphorus compounds, studies were undertaken to isolate bacteria capable of degrading O,O-dimethyl-O-(2-carbomethoxy-1-methylvinyl) phosphate (Phosdrin). Degradation of the insecticide was determined by gas chromatography, while decreased toxicity was confirmed by measuring cholinesterase activity with a recording pH stat. Of the thirty cultures screened, two showed 80-90% degradation of the original 20 ppm Phosdrin in 10 days, with a corresponding decrease in toxicity. One of the cultures was isolated from soil collected on Eglin Air Force Base, Florida, and the other as a laboratory contaminant. The bacteria were identified as two different strains of Bacillus megaterium. (Author)

Descriptors :   *INSECTICIDES, *PHOSPHATES, DEGRADATION, BACILLUS, GAS CHROMATOGRAPHY, ENZYMES, ORGANIC PHOSPHORUS COMPOUNDS.

Subject Categories : Toxicology
      Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE