Accession Number : AD0866922

Title :   Role of Sulfhydryl Groups in the Activity of Type A Botulinum Toxin,

Corporate Author : FORT DETRICK FREDERICK MD

Personal Author(s) : Knox, John N. ; Brown, William P. ; Spero, Leonard

Report Date : MAR 1970

Pagination or Media Count : 11

Abstract : Both crystalline botulinum toxin, type A (molecular weight 900,000) and a form of lower molecular weight (approx. 130,000) derived from it have four -SH groups and approximately one disulfide bridge per 100,000 grams of protein. Both forms were increasingly inactivated over a 24-hour period to one-tenth of the original toxicity by p-mercuribenzoate, but iodacetamide caused a smaller reduction and N-ethylmaleimide had no effect at all. Reversal of the p-mercuribenzoate inactivation by reduced glutathione could not be achieved. It is concluded that reaction of -SH groups may affect conformational stability of botulinum toxin, but these groups are not an integral part of its active center. (Author)

Descriptors :   *AMINO ACIDS), (*CLOSTRIDIUM BOTULINUM, TOXINS AND ANTITOXINS), (*TOXINS AND ANTITOXINS, DISEASES, FOOD POISONING, TOXICITY, MERCURY COMPOUNDS, IODINE COMPOUNDS, STABILITY, THIOLS.

Subject Categories : Biochemistry
      Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE